Further studies on the factor in lung-conditioned medium stimulating granulocyte and monocyte colony formation in vitro

✍ Scribed by J. W. Sheridan; D. Metcalf; E. R. Stanley

• Book ID: 102882434
• Publisher: John Wiley and Sons
• Year: 1974
• Tongue: English
• Weight: 981 KB
• Volume: 84
• Category: Article
• ISSN: 0021-9541
• DOI: 10.1002/jcp.1040840117

✦ Synopsis

Abstract

The CSF's extracted from 6‐hour “in vivo” post‐endotoxin mouse lung tissue and 48‐hour “in vitro” lung‐conditioned medium appear to be glyco‐proteins, for like human urinary CSF they were inactivated by proteases and altered in charge but not inactivated by sialidase.

Neither endotoxin injection nor length of in vitro incubation significantly affected the apparent S~20 W~ (approximately 2.OS) of the bulk of the CSF in mouse lung‐conditioned medium. The lower apparent molecular weight of CSF in mouse lung‐conditioned medium contrasted with that of monkey lung‐conditioned medium (S~20 W~ approximately 3.3S). CSF's in both monkey and mouse lung‐conditioned media exhibited a much higher apparent MW on gel nitration than one would expect from their sedimentation behavior, a characteristic generally observed with most unpurified CSF preparations.

Mouse lung‐conditioned medium CSF was antigenically distinguishable from other CSF's. Failure to demonstrate this form of CSF in the tissue serum or urine of normal or endotoxin‐injected mice suggests that this type of CSF might normally lack in vivo significance.