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Further studies by immunofluorescence of the monoclonal antibodies associated with experimental non-A, non-B hepatitis in chimpanzees and their relation to D hepatitis

✍ Scribed by Yohko K. Shimizu; Robert H. Purcell; John L. Gerin; Stephen M. Feinstone; Yasushi Ono; Toshio Shikata


Publisher
John Wiley and Sons
Year
1986
Tongue
English
Weight
519 KB
Volume
6
Category
Article
ISSN
0270-9139

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✦ Synopsis


To further investigate the specificity of the monoclonal antibodies (48-1 and S-1) associated with non-A, non-B hepatitis, extensive immunofluoreecence studies were performed on liver biopsy specimens from chimpanzees with experimental hepatitis A, B, non-A, non-B or 6, or from normal chimpanzees. Both 48-1 and S-1 antibodies reacted in the same manner with liver biopsy specimens from 47 of 50 (94%) chimpanzees with acute or chronic non-A, non-B hepatitis and 15 of 18 (83%) chimpanzees with type D hepatitis.

Examinations of serial liver biopsy specimens revealed that the duration of expression of the antigen reacting with the antibodies in hepatocytes of chimpanzees infected with non-A, non-B viruses appeared to be longer than that of chimpanzees infected with the hepatitis b-virus.

By thin-section electron microscopy, the presence of the microtubular aggregates, identical to those previ-