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Further evidence that the ribosomal 30S proteins S3, S5, S9, S11, S12, and S18 possess specific 16S RNA binding sites

✍ Scribed by Hochkeppel, Heinz-Kurt ;Craven, Gary R.

Publisher: Springer
Year: 1977
Tongue: English
Weight: 414 KB
Volume: 153
Category: Article
ISSN: 0026-8925
DOI: 10.1007/bf00431597

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✦ Synopsis

In this communication we demonstrate the site specificity of these proteins. Binding curves of the individual proteins with acetic acid-urea 16S RNA show that the binding of all six proteins to the RNA reaches a plateau at 0.3-0.97 copies per 16S RNA molecule. No significant binding of these proteins to classical phenol extracted 16S RNA is observed, with the exception of S 13 which binds 0.2 copies of protein per molecule of 16S RNA. Specificity of binding of these proteins is also demonstrated in "chase" experiments. The site specificity of individual [3H]-labeled 30S proteins bound to 16S RNA is tested by the addition of non-radioactive 30S total protein to the reaction mixture.

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