Further characterization of Artocarpus lakoocha lectin (artocarpin) purified using rivanol
✍ Scribed by Bishnu P. Chatterjee; Hafiz Ahmed; Sunanda Chowdhury
- Book ID
- 102993556
- Publisher
- Elsevier Science
- Year
- 1988
- Tongue
- English
- Weight
- 927 KB
- Volume
- 180
- Category
- Article
- ISSN
- 0008-6215
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✦ Synopsis
The a-D-galactosyl-binding lectin (artocarpin; mol. wt., 70,000) from Artocarpus lakoocha seeds has been purified using rivanol (6,9-diamino-2-ethoxyacridine lactate) and shown to be homogeneous by poly(acrylamide) disc gel electrophoresis and immunoelectrophoresis. It agglutinated human and several normal and enzyme-treated animal erythrocytes, and bound to rat lymphocytes and mouse ascites cells. The lectin is a metalloprotein with two sugar exothermic combining sites per lectin molecule of four subunits, and is inhibited by cY-glycosides of n-Gal/o-GalNAc with the cu-glycoside of P-o-Gal-(l-+3)-p-GalNAc being the most potent. Moruceae is specific for a-D-galactopyranoside and 2-acetamido-2-deoxy-a-Dgalactopyranoside residues is. Chuba and Kuhns reported16 the relatively good in-*Presented at the 4th European Carbohydrate Symposium,