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Further characterization, isolation and identification of the epithelial cell-surface antigen defined by monoclonal antibody aua1

โœ Scribed by H. Durbin; N. Rodrigues; W. F. Bodmer


Publisher
John Wiley and Sons
Year
1990
Tongue
French
Weight
462 KB
Volume
45
Category
Article
ISSN
0020-7136

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โœฆ Synopsis


The human epithelial antigen recognized by monoclonal antibody (MAb) AUAl has been characterized as a cell-surface glycoprotein. It has been isolated from human colonic mucosa by AUAl affinity separation. N-terminal peptide sequence of this purified material has revealed a I7 amino acid sequence which identifies it with one of a group of similar epithelial/ tumour-associated glycoproteins defined by a variety of MAbs. Using the polymerase chain reaction to map the gene encoding this antigen, our previous AUAl antigen assignment to chromosome 1 has been confirmed.

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