✦ LIBER ✦
Furan based cyclic homo-oligopeptides bind G-quadruplex selectively and repress c-MYC transcription
✍ Scribed by Tani Agarwal; Saumya Roy; Tushar Kanti Chakraborty; Souvik Maiti
- Book ID
- 104004555
- Publisher
- Elsevier Science
- Year
- 2010
- Tongue
- English
- Weight
- 390 KB
- Volume
- 20
- Category
- Article
- ISSN
- 0960-894X
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✦ Synopsis
We demonstrate the synthesis and selective binding of two novel furan based tricyclic homo-oligopeptides to G-quadruplex and using Real Time PCR show its repressive effect on c-MYC transcription. CD spectroscopy and FRET melting studies show that these ligands can induce G-quadruplex structures in the G rich 22 mer c-MYC DNA sequence and further stabilize the structure. Using real time polymerase chain reaction we observed that up to 70% downregulation of c-MYC transcripts upon ligand treatment in HeLa cells.