Fundamental cryobiology of rat immature and mature oocytes: Hydraulic conductivity in the presence of Me2SO, Me2SO permeability, and their activation energies

✍ Scribed by Agca, Y.; Liu, J.; Critser, E.S.; Critser, J.K.

• Book ID: 101226977
• Publisher: John Wiley and Sons
• Year: 2000
• Tongue: English
• Weight: 188 KB
• Volume: 286
• Category: Article
• ISSN: 0022-104X
• DOI: 10.1002/(sici)1097-010x(20000401)286:5<523::aid-jez10>3.0.co;2-9

✦ Synopsis

The hydraulic conductivity in the presence of dimethyl sulfoxide Me 2 SO (L p Me SO 2 ), Me 2 SO (P Me SO 2

) permeability and reflection coefficient (σ) of immature (germinal vesicle; GV) and mature (metaphase II; MII) rat oocytes were determined at various temperatures. A temperature controlled micropipette perfusion technique was used to conduct experiments at five different temperatures (30, 20, 10, 4, and -3°C). Kedem and Katchalsky membrane transport theory was used to describe the cell volume kinetics. The cell volumetric changes of oocytes were calculated from the measurement of two oocyte diameters, assuming a spherical shape. The activation energies (E a ) of L p Me SO 2 and P Me SO 2 were calculated using the Arrhenius equation. Activation energies of L p Me SO 2 for GV and MII oocytes were 34.30 Kcal/mol and 16.29 Kcal/mol, respectively; while the corresponding E a s of P Me SO 2 '94; Karlsson et al., '96) and GV stage oocytes (Candy et al., '94). Cryopreservation of rat embryos