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Functionalization of niobium electrodes for the construction of impedimetric biosensors

✍ Scribed by S. Helali; A. Abdelghani; I. Hafaiedh; C. Martelet; M.I. Prodromidis; T. Albanis; N. Jaffrezic-Renault

Publisher: Elsevier Science
Year: 2008
Tongue: English
Weight: 386 KB
Volume: 28
Category: Article
ISSN: 0928-4931
DOI: 10.1016/j.msec.2007.10.078

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✦ Synopsis

This paper describes the development of an impedimetric immunosensor, based on niobium/niobium oxide (Nb/NbOxHy) electrodes, for the detection of atrazine. Niobium oxide was anodically formed onto niobium electrodes at 25 V in 1 M H 2 SO 4 . Hydrous oxide layers were then silanized with APTES, and using glutaraldehyde as a cross linker, Fab fragment k47 antibody was covalently immobilized onto the surface of the electrodes. Electrochemical impedance spectroscopy (EIS) was used to characterize the building-up of the immunosensors as well as the binding of atrazine to its specific antibody. In presence of ferricyanide redox species and under a cathodic polarization voltage (-1.2 V versus SCE), the relationship between the concentration of atrazine and the change of the electron transfer resistance value was studied.

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