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Functionalization of Multi-Walled Carbon Nanotubes and their Application for Selective Isolation of Acidic Proteins

✍ Scribed by Zhuo Du; Yong-Liang Yu; Jian-Hua Wang

Publisher
John Wiley and Sons
Year
2009
Tongue
English
Weight
350 KB
Volume
9
Category
Article
ISSN
1616-5187

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✦ Synopsis

Abstract

A micro‐column packed with PDDA‐wrapped MWNTs in sequential injection system facilitates selective sorption of acidic protein species. Proteins adsorbed onto the PDDA‐MWNT composites are afterwards collected by elution with a citrate buffer as stripping reagent. With a sample loading volume of 2.0 mL and an eluent volume of 200 µL, a retention efficiency of 100% and a recovery of 90% are achieved for BSA in the range 6–120 µg, resulting in an enrichment factor of 14. A sampling frequency of 15 h^−1^ is achieved, along with a precision of 4.5% at 25 µg · mL^−1^ BSA. The practical applicability of this system is demonstrated by processing human whole blood for successive isolation of acidic proteins.

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