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Functional study of Lactococcus lactis RNase III in Escherichia coli

✍ Scribed by Djamel Drider; Alexander Bolotine; Pierre Renault; Hervé Prévost


Book ID
114121990
Publisher
Elsevier Science
Year
2002
Tongue
English
Weight
124 KB
Volume
47
Category
Article
ISSN
0147-619X

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Escherichia coli rnh mutants were isolated using localized mutagenesis and selective measurements of RNase H activity in mutagenized cell extracts with [3H]poly(rC) X poly(dG) as substrate. RNase H activity in extracts of one mutant, ON152 (rnh-91), was undetectable (less than 0.05% of that of wild-