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Functional Studies on Ribosomes Lacking Protein L1 from Mutant Escherichia coli

✍ Scribed by Alap R. SUBRAMANIAN; Eric R. DABBS


Book ID
115119614
Publisher
John Wiley and Sons
Year
1980
Tongue
English
Weight
518 KB
Volume
112
Category
Article
ISSN
1432-1327

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A battery of immunological tests were used to investigate mutants which had been determined as lacking one or two ribosomal proteins on the basis of two-dimensional polyacrylamide gels. Proteins which were confirmed as missing from the ribosome in one or more mutants were large subunit proteins L1,

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Proteins S4 and S12 were isolated from ribosomes of three mutants of Escherichia coli in which dependence on streptomycin caused by alteration in protein S12 is suppressed by an altered protein S4. Proteinchemical studies on the mutant proteins gave the following results: Proteins S12 from all three