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Functional studies on rat-liver isoferrints

โœ Scribed by Amyra Treffry; Pamela J. Lee; Pauline M. Harrison


Book ID
113264685
Publisher
Elsevier Science
Year
1984
Tongue
English
Weight
574 KB
Volume
785
Category
Article
ISSN
0167-4838

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3-Hydroxykynureninase was purified from rat liver. The Michaelis constants for L-kynurenine and L-3-hydroxykynurenine were determined to be 2.33 X 10(-4)M and 6.85 X 10(-5)M, respectively, at pH 8.41 and 37 degrees. With L-kynurenine as substrate, the enzyme was competitively inhibited by L-alanine,