Functional similarities of AeEαIa molecules as determined by analysis with T-cell clones
✍ Scribed by Barbara N. Beck; Anthony J. Infante; C. Garrison Fathman
- Book ID
- 104732930
- Publisher
- Springer-Verlag
- Year
- 1982
- Tongue
- English
- Weight
- 458 KB
- Volume
- 15
- Category
- Article
- ISSN
- 0093-7711
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✦ Synopsis
Recognition of AeE alpha Ia antigens at the functional level was investigated using T-cell clones. The reactivities of an alloreactive and an antigen-reactive clone, both of which recognized AeE alpha Ia molecules, were compared on a panel of stimulator/antigen-presenting cells of various genotypes. The two clones recognized all tested AebE alpha x Ia molecules, where x is a haplotype capable of expressing an Ia.7-bearing E alpha polypeptide. Ia antigen recognition by either clone could be inhibited by the monoclonal antibody Y-17, which recognizes a combinatorial serologic determinant on certain AeE alpha molecules. There were no differences in the recognition of Ia by the alloreactive versus the antigen-reactive clone, suggesting that Ia antigens are recognized by the two clones in a fundamentally similar way. The recognition of these various Ia molecules by the two cloned T-cell lines provides evidence that the E alpha polypeptides from H-2 haplotypes k, d, r, and u are functionally indistinguishable.