Functional significance of conserved amino acid residues

✍ Scribed by Dr. Anthony R. Poteete; Dale Rennell; Suzanne E. Bouvier

• Book ID: 105358628
• Publisher: John Wiley and Sons
• Year: 1992
• Tongue: English
• Weight: 280 KB
• Volume: 13
• Category: Article
• ISSN: 0887-3585
• DOI: 10.1002/prot.340130104

✦ Synopsis

Abstract

A systematic study of single amino acid substitutions in bacteriophage T4 lysozyme permitted a test of the concept that conserved amino acid residues are more functionally important than nonconserved residues. Substitutions of amino acid residues that are conserved among five bacteriophage‐encoded lysozymes were found to lead more frequently to loss of function than substitutions of nonconserved residues. Of 163 residues tested, only 74 (45%) are sensitive to at least one substitution; however, all 14 residues that are fully conserved are sensitive to substitutions. © 1992 Wiley‐Liss, Inc.