Functional replacement of yeast flavocytochrome b2 with bacterial l-lactate dehydrogenase
โ Scribed by Hiroaki Sakai; Kazuhiro Nagahama; Hisataka Taguchi; Takashi Akamatsu; Shigeru Morimura; Kenji Kida; Masayoshi Matsuoka
- Book ID
- 113683368
- Publisher
- Elsevier Science
- Year
- 2010
- Tongue
- English
- Weight
- 233 KB
- Volume
- 110
- Category
- Article
- ISSN
- 1389-1723
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๐ SIMILAR VOLUMES
## Abstract Flavocytochrome __b~2~__ or Lโlactate dehydrogenase from yeasts catalyzes the oxidation of Lโlactate at the expense of monoelectronic acceptors such as cytochrome __c__, its physiological partner. When incubated in the presence of both Lโlactate and a keto acid, the enzyme catalyzes a t
## Abstract The family of FMNโdependent, ฮฑโhydroxy acidโoxidizing enzymes catalyzes substrate dehydrogenation by a mechanism the first step of which is abstraction of the substrate ฮฑโproton (soโcalled carbanion mechanism). For flavocytochrome __b__~2~ and lactate oxidase, it was shown that once on