Secretory production of recombinant huma
โ
Hiroshi Nakakubo; Hajime Fukuyama; Masahide Nakajima; Teruaki Imada; Shusei Uno;
๐
Article
๐
2000
๐
John Wiley and Sons
๐
English
โ 140 KB
๐ 1 views
We succeeded in expressing in a Pichia pastoris (P. pastoris) host a cDNA encoding a mature human chymase (h-chymase) which was secreted directly into the culture medium. Recombinant human heart chymase (rh-chymase) was purified from the culture medium via a single one-step heparin-agarose column ch