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Functional over-expression of the Stm1 protein, a G-protein-coupled receptor, inSchizosaccharomyces pombe

✍ Scribed by Kyung-Sook Chung; Dong-Uk Kim; Sung-Woo Ryoo; Eun-Jung Kang; Misun Won; Lila Kim; Young-Joo Jang; Pil-Jae Maeng; Sei-Chang Kim; Hyang-Sook Yoo; Kwang-Lae Hoe


Book ID
110418900
Publisher
Springer Netherlands
Year
2003
Tongue
English
Weight
176 KB
Volume
25
Category
Article
ISSN
0141-5492

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Engineering a G protein-coupled receptor
✍ AimΓ©e Martin; Marjorie Damian; Michel Laguerre; Joseph Parello; Bernard Pucci; L πŸ“‚ Article πŸ“… 2009 πŸ› Cold Spring Harbor Laboratory Press 🌐 English βš– 253 KB

## Abstract Structural characterization of membrane proteins is hampered by their instability in detergent solutions. We modified here a G protein‐coupled receptor, the BLT1 receptor of leukotriene B~4~, to stabilize it __in vitro__. For this, we introduced a metal‐binding site connecting the third