Functional mutants of yeast alcohol dehydrogenase affecting kinetics, cellular redox balance, and electrophoretic mobility
✍ Scribed by Christopher Wills; Julia Phelps
- Book ID
- 104783824
- Publisher
- Springer
- Year
- 1978
- Tongue
- English
- Weight
- 921 KB
- Volume
- 16
- Category
- Article
- ISSN
- 0006-2928
No coin nor oath required. For personal study only.
✦ Synopsis
Repeated selection of petite (respiratorily incompetent) Saccharomyces cerevisiae on medium containing allyl alcohol, both on plates and in the turbidostat, results in mutants with a remarkably similar response. Most of the mutations affect the constitutive alcohol dehydrogenase, resulting in enzymes with a cathodal shift in electrophoretic mobility, and none shows a significant anodal shift. The genetics, kinetics, and physiological effect of three of the mutants have been investigated in detail, and while all confer resistance to allyl alcohol through a shift in the NAD/NADH ratio, they do so in slightly different ways. The potential of this system for exploring the range of short-term adaptations open to this organism is discussed.