𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Functional measurement of local proteolytic activity in living cells of invasive and non-invasive tumors

✍ Scribed by Thomas Ludwig; Sylvia Püttmann; Helga Bertram; Lars Tatenhorst; Werner Paulus; Hans Oberleithner; Volker Senner

Publisher
John Wiley and Sons
Year
2004
Tongue
English
Weight
557 KB
Volume
202
Category
Article
ISSN
0021-9541

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

Proteolytic cleavage of extracellular matrix (ECM) and disruption of tissue architecture are fundamental features of tumor cell invasion. The proteolytic activity is focused in close proximity to the tumor cells. Here, we describe the possibility to quantify local proteolytic activity in the microenvironment of larger cell populations by the electrical resistance breakdown assay. The assay utilizes the transepithelial electrical resistance (TEER) of an epithelial monolayer as a sensitive indicator of monolayer integrity and permeability. Local destruction of ECM by single tumor cells was demonstrated by a second assay, based on a fluorescent matrix coating on cover slides. Local digestion of the matrix results in a reduction of fluorescence. Primary cells derived from high and low grade brain tumors as well as established cell lines of malignant gliomas and non‐neural tumors of different origin (melanoma, cervical carcinoma, and breast carcinoma) were compared. Differences in proteolytic activity between tumor entities were demonstrated in both assays. Primary cells of high grade gliomas and cell lines showed TEER breakdown and local matrix destruction, while low grade brain tumors lacked matrix disintegration and disruption of cell monolayers. Taken together, both assays are capable of demonstrating local proteolytic activity and thus are versatile tools for distinguishing high and low invasive tumor cells with a potential application as diagnostic and prognostic markers in clinical investigations. The advantage of the matrix digestion assay is the requirement of only very low tumor cell numbers, whereas measurement of TEER enables precise quantification of local proteolytic processes in large and even heterogeneous tumor cultures. © 2004 Wiley‐Liss, Inc.

📜 SIMILAR VOLUMES

Local proteolytic activity in tumor cell
Local proteolytic activity in tumor cell invasion and metastasis
✍ Thomas Ludwig 📂 Article 📅 2005 🏛 John Wiley and Sons 🌐 English ⚖ 270 KB

## Abstract Proteolytic cleavage of extracellular matrix (ECM) is a critical regulator of many physiological and pathological events. It affects fundamental processes such as cell growth, differentiation, apoptosis and migration. Most proteases are produced as inactive proenzymes that undergo prote

Non-invasive analysis of cell cycle dyna
Non-invasive analysis of cell cycle dynamics in single living cells with Raman micro-spectroscopy
✍ Robin J. Swain; Gavin Jell; Molly M. Stevens 📂 Article 📅 2008 🏛 John Wiley and Sons 🌐 English ⚖ 343 KB 👁 1 views

## Abstract Raman micro‐spectroscopy is a laser‐based technique which enables rapid and non‐invasive biochemical analysis of cells and tissues without the need for labels, markers or stains. Previous characterization of the mammalian cell cycle using Raman micro‐spectroscopy involved the analysis o

Activation of progelatinase A (MMP-2) by
Activation of progelatinase A (MMP-2) by neutrophil elastase, cathepsin G, and proteinase-3: A role for inflammatory cells in tumor invasion and angiogenesis
✍ Peter Shamamian; Jess D. Schwartz; Ben J.Z. Pocock; Sara Monea; David Whiting; S 📂 Article 📅 2001 🏛 John Wiley and Sons 🌐 English ⚖ 236 KB

## Abstract Gelatinase A (MMP‐2), a matrix metalloproteinase (MMP) involved in tumor invasion and angiogenesis, is secreted as an inactive zymogen (proMMP‐2) and activated by proteolytic cleavage. Here we report that polymorphonuclear neutrophil (PMN)‐derived elastase, cathepsin G, and proteinase‐3

Inhibition of cell proliferation, invasi
Inhibition of cell proliferation, invasion, tumor growth and metastasis by an oral non-antimicrobial tetracycline analog (COL-3) in a metastatic prostate cancer model
✍ Bal L. Lokeshwar; Marie G. Selzer; Bao-Qian Zhu; Norman L. Block; Lorne M. Golub 📂 Article 📅 2002 🏛 John Wiley and Sons 🌐 French ⚖ 460 KB

## Abstract Antibiotic forms of tetracycline exhibit antitumor activity in some tumor models. However, their low __in vivo__ efficacy and associated morbidity limit their long‐term application in cancer therapy. This report appraises the efficacy of doxycycline (DC) and non‐antimicrobial, chemicall