Functional heterogeneity of the 30S ribosomal subunit ofEscherichia coli
β Scribed by van Duin, J. ;van Knippenberg, P. H. ;Dieben, M. ;Kurland, C. G.
- Book ID
- 104819200
- Publisher
- Springer
- Year
- 1972
- Tongue
- English
- Weight
- 623 KB
- Volume
- 116
- Category
- Article
- ISSN
- 0026-8925
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The present article is concerned with ribosome assembly. However, this theme is really only a device which is introduced t o explore the functions of that two-thirds of the prokaryotic ribosome which is RNA. A close look at ribosome assembly turns out to be a convenient way t o approach this problem
Escherichia coli was depleted of ribosomes by a thermal shock at 47 degrees C which quantitatively destroyed the 30S ribosomal subunits. During recovery in minimal medium at 30 degrees C RNA is synthesized while protein synthesis resumes only after about 90 min. It is shown that lac mRNA is synthesi