✦ LIBER ✦

Functional expression of mammalian opioid receptors in insect cells and high-throughput screening platforms for receptor ligand mimetics

✍ Scribed by L. Swevers; E. Morou; N. Balatsos; K. Iatrou; Z. Georgoussi

Book ID: 105758064
Publisher: Springer
Year: 2005
Tongue: English
Weight: 258 KB
Volume: 62
Category: Article
ISSN: 1420-682X
DOI: 10.1007/s00018-005-4537-1

No coin nor oath required. For personal study only.

📜 SIMILAR VOLUMES

A Highly Specific Cell-Based High-Throug

A Highly Specific Cell-Based High-Throughput Screening Assay for Ligands of Cyclic Adenosine Monophosphate Receptor Protein in Gram-Negative Bacteria

✍ Wang, Hongxia; Silva, Anisia J.; Rasmussen, Lynn; White, E. Lucile; Benitez, Jor 📂 Article 📅 2013 🏛 Mary Ann Liebert 🌐 English ⚖ 217 KB

A Chimeric Reporter Gene Allowing for Cl

A Chimeric Reporter Gene Allowing for Clone Selection and High-Throughput Screening of Reporter Cell Lines Expressing G-Protein-Coupled Receptors

✍ Knut Kotarsky; Christer Owman; Björn Olde 📂 Article 📅 2001 🏛 Elsevier Science 🌐 English ⚖ 114 KB

Efficient screening of ligands interacting with Gprotein-coupled receptors is central for modern drug development. Here, we describe an optimized reporter vector primarily intended for use in reporter cell lines expressing such receptors. The construct consists of a synthetic enhancer containing 9؋

[Advances in Virus Research] Insect Viru

[Advances in Virus Research] Insect Viruses: Biotechnological Applications Volume 68 || Stably Transformed Insect Cell Lines: Tools for Expression of Secreted and Membrane‐anchored Proteins and High‐throughput Screening Platforms for Drug and Insecticide Discovery

✍ Douris, Vassilis 📂 Article 📅 2006 🏛 Elsevier ⚖ 858 KB

Histamine H4 receptor–RGS fusion protein

Histamine H4 receptor–RGS fusion proteins expressed in Sf9 insect cells: A sensitive and reliable approach for the functional characterization of histamine H4 receptor ligands

✍ Erich H. Schneider; Roland Seifert 📂 Article 📅 2009 🏛 Elsevier Science 🌐 English ⚖ 539 KB

A Reporter Gene Assay for High-Throughpu

A Reporter Gene Assay for High-Throughput Screening of G-Protein-Coupled Receptors Stably or Transiently Expressed in HEK293 EBNA Cells Grown in Suspension Culture

✍ Yves Durocher; Sylvie Perret; Eric Thibaudeau; Marie-Helene Gaumond; Amine Kamen 📂 Article 📅 2000 🏛 Elsevier Science 🌐 English ⚖ 184 KB

We describe in detail a robust, sensitive, and versatile functional assay for G-protein-coupled receptors (GPCRs) expressed in human embryonic kidney (HEK) 293-EBNA (Epstein-Barr virus nuclear antigen) (designated 293E) cells. The ability to grow these cells in suspension, in conjunction with the us

High-purity selection and maintenance of

High-purity selection and maintenance of gene expression in human neuroblastoma cells stably over-expressing GFP fusion protein: Application for opioid receptors desensitization studies

✍ Benjamin Aguila; Mikel Roussel; Philippe Jauzac; Stéphane Allouche 📂 Article 📅 2006 🏛 Elsevier Science 🌐 English ⚖ 359 KB