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Functional expression of horseradish peroxidase in Saccharomyces cerevisiae and Pichia pastoris

✍ Scribed by Morawski, B.; Lin, Z.; Cirino, P.; Joo, H.; Bandara, G.; Arnold, F. H.

Book ID
121853238
Publisher
Oxford University Press
Year
2000
Tongue
English
Weight
310 KB
Volume
13
Category
Article
ISSN
1741-0126

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Functional expression and stabilization
Functional expression and stabilization of horseradish peroxidase by directed evolution in Saccharomyces cerevisiae
✍ Birgit Morawski; Sara Quan; Frances H. Arnold πŸ“‚ Article πŸ“… 2001 πŸ› John Wiley and Sons 🌐 English βš– 287 KB

Biotechnology applications of horseradish peroxidase (HRP) would benefit from access to tailor-made variants with greater specific activity, lower K(m) for peroxide, and higher thermostability. Starting with a mutant that is functionally expressed in Saccharomyces cerevisiae, we used random mutagene