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Functional Expression of Horseradish Peroxidase in E. coli by Directed Evolution

✍ Scribed by Lin, Z. ;Thorsen, T. ;Arnold, F.H.

Book ID
109388353
Publisher
American Institute of Chemical Engineers
Year
2000
Tongue
English
Weight
13 KB
Volume
16
Category
Article
ISSN
8756-7938

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Functional expression and stabilization
Functional expression and stabilization of horseradish peroxidase by directed evolution in Saccharomyces cerevisiae
✍ Birgit Morawski; Sara Quan; Frances H. Arnold πŸ“‚ Article πŸ“… 2001 πŸ› John Wiley and Sons 🌐 English βš– 287 KB

Biotechnology applications of horseradish peroxidase (HRP) would benefit from access to tailor-made variants with greater specific activity, lower K(m) for peroxide, and higher thermostability. Starting with a mutant that is functionally expressed in Saccharomyces cerevisiae, we used random mutagene