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Functional expression of cytochrome P450s in Escherichia coli for the biotransformation of daidzein to produce ortho-specific monohydroxylation

✍ Scribed by Kwon-Young Choi; Changhyun Roh; Bishnu P. Pandey; Nahum Lee; Taejin Kim; Seungkee Kim; Byung-Gee Kim


Book ID
118472809
Publisher
Elsevier Science
Year
2008
Tongue
English
Weight
70 KB
Volume
136
Category
Article
ISSN
0168-1656

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The Mud(Aplac) operon fusion technique of Casadaban and Cohen (1979) was used to search for inducible functions specific to the RecF pathway of conjugal recombination. A fusion mutant of a recBC sbcB mutant which showed less than 1% of the normal level of recombination in Hfr crosses has been isolat