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Functional consequences of improved structural information on bacterial nucleods

โœ Scribed by Kellenberger, E.


Book ID
122846424
Publisher
Elsevier Science
Year
1991
Tongue
English
Weight
683 KB
Volume
142
Category
Article
ISSN
0923-2508

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## Abstract The engineering of lipase B from __Candida antarctica__ (CALB) by circular permutation has yielded over sixty hydrolase variants, and several show significantly improved catalytic performance. Here we report a detailed characterization of ten selected enzyme variants by kinetic and spec