Functional characterization of human Kindlin-2 core promoter identifies a key role of SP1 in Kindlin-2 transcriptional regulation

✍ Scribed by Ammad Aslam Khan; Takashi Shimokawa; Staffan Strömblad; Hongquan Zhang

• Book ID: 111490486
• Publisher: SP Versita
• Year: 2011
• Tongue: English
• Weight: 934 KB
• Volume: 16
• Category: Article
• ISSN: 1425-8153
• DOI: 10.2478/s11658-011-0028-6

✦ Synopsis

Abstract

Kindlin-2 is a recently identified FERM and PH domain containing integrin interacting protein. Kindlin-2 is ubiquitously expressed in normal tissues. So far, much effort has been spent exploring the functional aspects of Kindlin-2. However, the transcriptional regulation of Kindlin-2 has not yet been investigated. In this study we identified and functionally characterized the promoter of the human Kindlin-2 gene. We show that the core promoter of Kindlin-2 is a 39 base pair long GC rich fragment located −122/-83 upstream of the Kindlin-2 transcription start site. Functional characterization of this core promoter region by both in silico as well as in vitro/in vivo analysis shows that the transcription factor SP1 plays an important role in regulation of Kindlin-2 expression.