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Functional antagonism by a monoclonal antibody to digoxin in a test system of cultured rat heart myocytes

✍ Scribed by G. Wallukat; H. -U. Simon; W. -D. Müller; I. Wolf

Publisher: Springer
Year: 1996
Tongue: English
Weight: 350 KB
Volume: 160-161
Category: Article
ISSN: 0300-8177
DOI: 10.1007/bf00240040

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✦ Synopsis

A monoclonal antibody with a high affinity for digitoxin (KA = 0.50 nM) and digoxin (KA = 0.55 nM) was produced by somatic cell fusion. This antibody, designated 2A3(47), displayed little cross reactivity with other glycosides. In cultured rat heart myocytes, 2A3(47), antagonized the positive chronotropic effect exerted by digitoxin but did not alter that of ouabain. Our results suggest that this monoclonal antibody may prove to be useful in treating digoxin and digitoxin intoxication.

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