Functional and phenotypical characteristics of hepatic NK-like T cells in NK1.1-positive and -negative mouse strains
✍ Scribed by Kouichi Shinohara; Yoshinori Ikarashi; Hidenori Maruoka; Michio Miyata; Takashi Sugimura; Masaaki Terada; Hiro Wakasugi
- Publisher
- John Wiley and Sons
- Year
- 1999
- Tongue
- English
- Weight
- 101 KB
- Volume
- 29
- Category
- Article
- ISSN
- 0014-2980
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✦ Synopsis
We previously reported and partially characterized a unique monoclonal antibody (mAb), U5A2-13, which recognizes a T cell subset similar to NK1.1 + T cells, not only in NK1.1positive mouse strains but also in NK1.1-negative strains. In NK1.1-positive C57BL/6 mice, U5A2-13 + TCR § g + cells produced abundant IL-4 as well as extremely high levels of IFN-+ upon CD3 cross-linking, but this did not occur with U5A2-13 -TCR § g + cells. In NK1.1negative C3H/He mice, U5A2-13 + TCR § g + cells produced high levels of IL-4 and IFN-+ upon CD3 cross-linking, but this was not observed with U5A2-13 -TCR § g + cells. To the best of our knowledge, this is the first direct evidence of the presence of NK-like T cells defined phenotypically by U5A2-13 mAb and functionally by IL-4/IFN-+ production in NK1.1-negative mouse strains. We also demonstrated that U5A2-13 -NK1.1 + T cells and U5A2-13 + NK1.1 -T cells in C57BL/6 mice could produce both IL-4 and IFN-+ . In addition, V g 8 or V g 7 usage by U5A2-13 + NK1.1 -T cells was lower than that by U5A2-13 + NK1.1 + T cells, but remained higher than that by U5A2-13 -NK1.1 -T cells. Based on the present results, U5A2-13 mAb appears to be a valuable tool in the study of NK-like T cells.