✦ LIBER ✦
Functional Analysis of MycE and MycF, Two O-Methyltransferases Involved in the Biosynthesis of Mycinamicin Macrolide Antibiotics
✍ Scribed by Shengying Li; Yojiro Anzai; Kenji Kinoshita; Fumio Kato; David H. Sherman
- Publisher
- John Wiley and Sons
- Year
- 2009
- Tongue
- English
- Weight
- 431 KB
- Volume
- 10
- Category
- Article
- ISSN
- 1439-4227
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✦ Synopsis
Abstract
Mg motors: We characterized the in vitro function of MycE and MycF, two O‐methyltransferases involved in the biosynthesis of mycinamicin antibiotics. Each enzyme was confirmed to be an S‐adenosyl‐L‐methionine (SAM)‐dependent deoxysugar methyltransferase. Their optimal activities require the presence of Mg^2+^. With the reconstituted in vitro assays, the order of mycinamicin VI→III→IV in the post‐PKS (polyketide synthase) tailoring pathway of mycinamicin was unambiguously determined.magnified image