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Functional 70S hybrid ribosomes from blue-green algae and bacteria

โœ Scribed by John E. Gray; Diane S. Herson


Book ID
104770026
Publisher
Springer
Year
1976
Tongue
English
Weight
446 KB
Volume
109
Category
Article
ISSN
0302-8933

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โœฆ Synopsis


Hybrid 70S ribosomes were produced by combing Anacystis nidulans and Escherichia coli 30S and 50S subunits. Both the A. nidulans 30SE. coli 50S and E. coli 30S- A. nidulans 50S hybrids were functional in synthesizing protein when tested in a standard in vitro amino acid incorporating system. Both 70S hybrids were inhibited by streptomycin but the degree of inhibition was dependent upon the source of the 30S subunit. The ability to form functional 70S ribosomes from subunits of blue-green algae and bacteria is further evidence of the procaryotic nature of blue-greens and of the functional homology of the two protein synthesizing systems.


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The complete nucleotide sequence of a 16
โœ Tomioka, Noboru ;Sugiura, Masahiro ๐Ÿ“‚ Article ๐Ÿ“… 1983 ๐Ÿ› Springer ๐ŸŒ English โš– 393 KB

The complete nucleotide sequence of a 16S ribosomal RNA gene from a blue-green alga, Anacystis nidulans, has been determined. Its coding region is estimated to be 1,487 base pairs long, which is nearly identical to those reported for chloroplast 16S rRNA genes and is about 4% shorter than that of th