𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Function and regulation of cytochrome P-450 in alkane-assimilating yeast

✍ Scribed by W. -H. Schunck; S. Mauersberger; J. Huth; P. Riege; H. -G. Müller

Publisher
Springer
Year
1987
Tongue
English
Weight
532 KB
Volume
147
Category
Article
ISSN
0302-8933

No coin nor oath required. For personal study only.

✦ Synopsis

A method for selective and reversible inhibition of the alkane hydroxylating cytochrome P-450 in growing yeast cells was developed. Using low concentrations of carbon monoxide in a specially adapted small-scale fermentor system a complete inhibition of the first step of alkane degradation was obtained, obviously without influencing further metabolic steps. Thus fatty alcohols which were shown to be the immediate products of the cytochrome P-450 catalyzed reaction were utilized with unchanged rates by yeast cultures blocked with carbon monoxide in alkane degradation.

Moreover, significant differences were found in the pO2dependencies of utilization of n-alkanes, fatty alcohols and further intermediates. It was concluded, that the alkane hydroxylating cytochrome P-450 system is distinguished by the lowest oxygen affinity among the oxygen-activating enzyme systems involved in alkane assimilation.

📜 SIMILAR VOLUMES

Cloning and characterization of ann-alka
Cloning and characterization of ann-alkane-inducible cytochrome P450 gene essential forn-decane assimilation byYarrowia lipolytica
✍ Iida, Toshiya; Ohta, Akinori; Takagi, Masamichi 📂 Article 📅 1998 🏛 John Wiley and Sons 🌐 English ⚖ 408 KB 👁 2 views

A gene encoding cytochrome P450 involved in n-alkane utilization was cloned from an n-alkane assimilating yeast, Yarrowia lipolytica CX161-1B. The RT-PCR was performed on the mRNA prepared from the cells grown on n-alkane as a template using degenerated PCR primers designed for the conserved amino a

Coexpression in yeast of Taxus cytochrom
Coexpression in yeast of Taxus cytochrome P450 reductase with cytochrome P450 oxygenases involved in Taxol biosynthesis
✍ Stefan Jennewein; Hangil Park; JingHong M. DeJong; Robert M. Long; Arthur P. Bol 📂 Article 📅 2005 🏛 John Wiley and Sons 🌐 English ⚖ 545 KB

## Abstract To maximize redox coupling efficiency with recombinant cytochrome P450 hydroxylases from yew (__Taxus__) species installed in yeast for the production of the anticancer drug Taxol, a cDNA encoding NADPH:cytochrome P450 reductase from __T. cuspidata__ was isolated. This single‐copy gene

Expression and regulation of cytochrome
Expression and regulation of cytochrome P450 enzymes in primary cultures of human hepatocytes
✍ Edward L. LeCluyse; Ajay Madan; Geraldine Hamilton; Kathy Carroll; Ryan DeHaan; 📂 Article 📅 2000 🏛 John Wiley and Sons 🌐 English ⚖ 339 KB 👁 1 views

The aim of this study was to test suitable culture conditions for maintaining normal cellular cytoarchitecture and inducibility of P450 enzymes in primary cultures of human hepatocytes by prototypical inducers. The selectivity and sensitivity of a sandwich culture system were determined by treating