Fumonisin B1 alters cell cycle progression and interleukin-2 synthesis in swine peripheral blood mononuclear cells

✍ Scribed by Daniela E. Marin; Marie-Estelle Gouze; Ionelia Taranu; Isabelle P. Oswald

• Publisher: John Wiley and Sons
• Year: 2007
• Tongue: English
• Weight: 270 KB
• Volume: 51
• Category: Article
• ISSN: 1613-4125
• DOI: 10.1002/mnfr.200700131

✦ Synopsis

Abstract

Fumonisin B~1~ (FB~1~) is a mycotoxin produced by Fusarium verticillioides, a fungus that commonly contaminates maize. In the present study, we investigated the effects of FB~1~ on swine peripheral blood mononuclear cells (PBMC) by measuring cell proliferation, cell cycle progression and interleukin (IL)‐2 production. Forty‐eight hours after treatment in vitro, FB~1~ induced a decrease of PBMC proliferation as measured by cell counting and dehydrogenase enzyme activity. This effect was observed starting with 10 μM FB~1~. The effect of FB~1~ on cell cycle progression was analyzed by flow cytometry. Incubation of PBMC with FB~1~ increased the percentage of cells blocked in G0/G1 phase of the cell cycle. Treatment with 200 μM FB~1~ induces a high blockade of the cell cycle, with 92.4% of cells in G0/G1 phase. This blockade was observed in all lymphocyte subsets tested (CD2^+^, CD4^+^, CD8^+^ and Ig^+^) as evidenced by dual staining for DNA and membrane surface molecules. A significant decrease of IL2 production was also observed in the supernatants of ConA stimulated PBMC treated with 100 or 200 μM FB~1~. In conclusion, these data suggest that FB~1~ may affect immune functions by inhibiting lymphocyte proliferation and IL2 production, suggesting a possible role of FB~1~ exposure during infectious disease and cancer.