Fully Automated Two-dimensional Capillary Electrophoresis for High Sensitivity Protein Analysis
β Scribed by Michels, D. A.
- Book ID
- 115537056
- Publisher
- The American Society for Biochemistry and Molecular Biology
- Year
- 2001
- Tongue
- English
- Weight
- 166 KB
- Volume
- 1
- Category
- Article
- ISSN
- 1535-9476
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
Capillary electrophoresis/time-of-flight mass spectrometry(CE/TOFMS) has been used for analysis of in-gel digests of protein spots excised from two-dimensional sodium dodecyl sulfate polyacrylamide gel electrophoresis (2-D SDS-PAGE). An off-line purification and preconcentration procedure with a Zip
## Abstract A twoβdimensional capillary electrophoresis platform, combining isoelectric focusing (IEF) and capillary zone electrophoresis (CZE), was established on a microchip with the channel width and depth as 100 ΞΌm and 40 ΞΌm, respectively. With polyacrylamide as permanent coating, EOF in the mi