Fully automated, high yielding production of N-succinimidyl 4-[18F]fluorobenzoate ([18F]SFB), and its use in microwave-enhanced radiochemical coupling reactions
✍ Scribed by Peter J. H. Scott; Xia Shao
- Publisher
- John Wiley and Sons
- Year
- 2010
- Tongue
- French
- Weight
- 251 KB
- Volume
- 53
- Category
- Article
- ISSN
- 0022-2135
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✦ Synopsis
Abstract
A General Electric Medical Systems (GEMS) Tracerlab FX~FN~ fluorine‐18 synthesis module has been reconfigured to allow rapid (45 min), fully automated production of N‐succinimidyl 4‐[^18^F]fluorobenzoate ([^18^F]SFB) using the established three‐step, one‐pot synthesis procedure. Purification is by sep‐pak only and [^18^F]SFB is routinely obtained in 38% non‐decay corrected yield,>1 Ci/µmol specific activity, and >95% radiochemical purity (n=20). Moreover, this report includes our preliminary research efforts into improving peptide coupling reactions with [^18^F]SFB using microwave‐enhanced radiochemistry. Reaction times can be reduced by>90%, when compared with traditional thermal reactions, with no significant effect on radiochemical reaction yield. Copyright © 2010 John Wiley & Sons, Ltd.