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Full-term development of mouse blastomere nuclei transplanted into enucleated two-cell embryos

✍ Scribed by Tsunoda, Y. ;Yasui, T. ;Shioda, Y. ;Nakamura, K. ;Uchida, T. ;Sugie, T.

Publisher: John Wiley and Sons
Year: 1987
Tongue: English
Weight: 434 KB
Volume: 242
Category: Article
ISSN: 0022-104X
DOI: 10.1002/jez.1402420205

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✦ Synopsis

The nuclei from four-and eight-cell mouse embryos were transplanted into enucleated two-cell embryos. It was found that such embryos not only developed to the blastocyst stage in vitro (72% and 35%), but also developed to full term (22% and 8%) after transfer to recipient mice. However, development of embryos which contained nuclei from the inner cell mass was not observed. Since the development of enucleated zygotes which contain advanced nuclei is limited (the present study;

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The developmental ability of enucleated mouse oocytes reconstituted between the nucleus from 4-cell mouse embryos at different cell stages and recipient cytoplasms at different conditions, and the developmental ability of oocytes receiving nuclei from compacted morulae were examined. The highest dev