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Full proton NMR assignment of the reduced form of a repeat unit tetrasaccharide derived by keratanase digestion of keratan sulphate

✍ Scribed by T. N. Huckerby; J. M. Dickenson; I. A. Nieduszynski

Publisher: John Wiley and Sons
Year: 1990
Tongue: English
Weight: 502 KB
Volume: 28
Category: Article
ISSN: 0749-1581
DOI: 10.1002/mrc.1260280910

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✦ Synopsis

Abstract

The full assignment of the ambient‐temperature high‐field ^1^H NMR spectrum for the reduced form of a repeat‐unit β‐linked tetrasaccharide derived by keratanase digestion from keratan sulphate, GlcNAc(6‐SO~4~)^1^^3^Gal(6‐SO~4~)^1^^4^GlcNAc(6‐SO~4~)^1^^3^Gal‐ol, was achieved using two‐dimensional COSY and RELAYED‐COSY experiments. The problem of the choice of the measurement temperature for ^1^H NMR spectra from keratan sulphate oligomers and polymers is discussed. A modified protocol for the preparation of keratanase‐derived oligosaccharides is proposed.

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13C NMR spectroscopy of keratan sulphate

13C NMR spectroscopy of keratan sulphates: Assignments for the reduced form of a repeat unit tetrasaccharide derived from keratan sulphate by keratanase digestion and partial assignments for two fucosylated pentasaccharides

✍ Thomas N. Huckerby; John M. Dickenson; Gui-Hua Tai; Robert M. Lauder; Gavin M. B 📂 Article 📅 1993 🏛 John Wiley and Sons 🌐 English ⚖ 452 KB

## Abstract A full assignment of the 60°C ^13^C NMR spectrum for the reduced form of a repeat unit β‐linked tetrasaccharide derived by keratanase digestion from keratan sulphate, GlcNAc(6SO~4~)β^1–3^Gal(6SO~4~)β^1–4^GlcNAc(6SO~4~)β^1–3^‐Gal‐ol, was achieved via two‐dimensional C/H correlation and ^