✦ LIBER ✦

Full genomic amplification and subtyping of influenza A virus using a single set of universal primers

✍ Scribed by Emi Inoue; Xiaofeng Wang; Yoshiaki Osawa; Katsunori Okazaki

Book ID: 108578388
Publisher: Center for Academic Publications, Japan
Year: 2010
Tongue: English
Weight: 176 KB
Volume: 54
Category: Article
ISSN: 0385-5600
DOI: 10.1111/j.1348-0421.2009.00193.x

No coin nor oath required. For personal study only.

📜 SIMILAR VOLUMES

Universal primer set for the full-length

Universal primer set for the full-length amplification of all influenza A viruses

✍ E. Hoffmann; J. Stech; Y. Guan; R. G. Webster; D. R. Perez 📂 Article 📅 2001 🏛 Springer Vienna 🌐 English ⚖ 445 KB

Amplification of human genomic DNA seque

Amplification of human genomic DNA sequences with polymerase chain reaction using a single oligonucleotide primer

✍ Liuying Luo; Eleftherios P. Diamandis 📂 Article 📅 1999 🏛 John Wiley and Sons 🌐 English ⚖ 157 KB 👁 1 views

We present two examples of exponential nucleic acid amplification with the polymerase chain reaction (PCR) in the presence of only one amplification primer. Cloning and sequencing of the PCR products generated by amplification of human genomic DNA revealed that the amplified sequence contained only

Amplification and typing of multiple cer

Amplification and typing of multiple cervical cancer-associated human papillomavirus DNAS using a single pair of primers

✍ H. Yoshikawa; T. Kawana; K. Kitagawa; M. Mizuno; H. Yoshikura; A. Iwamoto 📂 Article 📅 1990 🏛 John Wiley and Sons 🌐 French ⚖ 273 KB

Full-length genomic sequence of a hepati

Full-length genomic sequence of a hepatitis C virus genotype 2c isolate (BEBE1) and the 2c-specific PCR primers

✍ H. Nakao; H. Okamoto; H. Tokita; T. Inoue; H. Iizuka; G. Pozzato; S. Mishiro 📂 Article 📅 1996 🏛 Springer Vienna 🌐 English ⚖ 252 KB

Evaluation of a single-step multiplex RT

Evaluation of a single-step multiplex RT-PCR for influenza virus type and subtype detection in respiratory samples

✍ Saibal K. Poddar; Rowena Espina; David P. Schnurr 📂 Article 📅 2002 🏛 John Wiley and Sons 🌐 English ⚖ 46 KB

## Abstract A single‐step multiplex reverse transcription‐polymerase chain reaction (RT‐PCR) for the simultaneous detection of influenza virus type and subtypes was evaluated for its use in clinical specimens. One part of each specimen was tested using an established standard culture and/or monoclo

Optimized PCR amplification of influenza

Optimized PCR amplification of influenza A virus RNA using Tth DNA polymerase, incorporating uracil N glycosylase (UNG) in a single tube reaction

✍ Saibal K. Poddar; Mark H. Sawyer; James D. Connor 📂 Article 📅 1997 🏛 John Wiley and Sons 🌐 English ⚖ 77 KB 👁 1 views

An optimized reaction condition for amplification of influenza A virus RNA, by thermus thermophilus (Tth) DNA polymerase-based PCR, incorporating uracil N glycosylase (UNG) and dUTP in the reaction has been determined. dUTP could not be substituted for all dTTP sites when UNG was present in the reac