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Frontal affinity chromatography—mass spectrometry

✍ Scribed by Ng, Ella S M; Chan, Nora WC; Lewis, Darren F; Hindsgaul, Ole; Schriemer, David C


Book ID
109946238
Publisher
Nature Publishing Group
Year
2007
Tongue
English
Weight
361 KB
Volume
2
Category
Article
ISSN
1750-2799

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Frontal Affinity Chromatography Coupled
✍ Boyan Zhang; Monica M. Palcic; David C. Schriemer; Gerardo Alvarez-Manilla; Mich 📂 Article 📅 2001 🏛 Elsevier Science 🌐 English ⚖ 136 KB

Frontal affinity chromatography coupled online to mass spectrometry (FAC/MS) has previously been used to estimate binding constants for individual protein ligands present in mixtures of compounds. In this study FAC/MS is used to determine enzyme substrate kinetic parameters and binding constants for