✦ LIBER ✦

Frictional coefficients of multisubunit structures. II. Application to proteins and viruses

✍ Scribed by V. Bloomfield; K. E. Van Holde; W. O. Dalton

Publisher: Wiley (John Wiley & Sons)
Year: 1967
Tongue: English
Weight: 650 KB
Volume: 5
Category: Article
ISSN: 0006-3525
DOI: 10.1002/bip.1967.360050203

No coin nor oath required. For personal study only.

✦ Synopsis

The theory of Kirkwood for the translational frictional coefficients of structures composed of identical subunits has been extended in the previous paper t.o the case where nonidentical snbiinits are involved. In this paper, the t.heory is applied to particular proteins and viruses. It is found that t,he calculated sedimentation coefficients of various states of aggregation of the reversibly associating proteins hemocyanin and phycocyanin are in excellent agreement with experiment. The dimensions of the fibrinogen moleciile obtained from electron micrographs do not give good agreement bet,weeri calculated and experimental frictional coefficients. The frictional coefficient of tobacco mosaic virus calculated without explicit consideration of end effects is in good agreement with experiment if a hydrodynamic diameter of 180 A., corresponding to the maximum diameter from x-ray studies, is used. Agreement is also good for the faqt, sedimenting form of bacteriophage T2 and the protein ghosts of bacteriophage A ; but, the slow form of T2 and whole A have frictional coefficients considerably in excess of those calculated. Tail fiber confignration or head porosity are unable to account for the difference in sedimentat~ion coefficients between the fast and slow forms of T2.

1.19

📜 SIMILAR VOLUMES

Frictional properties of nonspherical mu

Frictional properties of nonspherical multisubunit structures. Application to tubules and cylinders

✍ J. A. McCammon; J. M. Deutch 📂 Article 📅 1976 🏛 Wiley (John Wiley & Sons) 🌐 English ⚖ 536 KB

## Abstract Methods are described for numerical calculation of the anisotropic components of the translational and rotational friction coefficient tensors and of the intrinsic viscosity for rigid multisubunit structures in dilute solution. The methods apply to assemblies of any shape, provided that

Epidemiologic applications of novel mole

Epidemiologic applications of novel molecular methods to detect and differentiate small round structured viruses (Norwalk-like viruses)

✍ Tamie Ando; Qi Jin; Jon R. Gentsch; Stephan S. Monroe; Jacqueline S. Noel; Scott 📂 Article 📅 1995 🏛 John Wiley and Sons 🌐 English ⚖ 794 KB

## Abstract The molecular epidemiology of a large, multi‐state outbreak of oyster‐associated gastroenteritis [Kohn et al. (1995): __Journal of the American Medical Association 273__:466–471. Dowell et al. (1995): __Journal of Infectious Diseases 171__:1497–1503.] was examined using new methods to d

In vitro labeling of proteins by reducti

In vitro labeling of proteins by reductive methylation: Application to proteins involved in supramolecular structures

✍ C. S. Heacock; B. W. Bernstein; A. S. Duhaiman; D. A. Amorese; J. R. Bamburg 📂 Article 📅 1982 🏛 John Wiley and Sons 🌐 English ⚖ 846 KB

## Abstract Actin and tropomyosin, purified from both muscle and brain, and α‐actinin, purified from muscle, have been labeled in vitro by reductive methylation to specific activities of greater than 10^5^ dpm/μg protein. Actin so modified bound DNase I and polymerized identically to unmodified act

Noninteracting local-structure model of

Noninteracting local-structure model of folding and unfolding transition in globular proteins. II. Application to two-dimensional lattice proteins

✍ Haruo Abe; Nobuhiro GŌ 📂 Article 📅 1981 🏛 Wiley (John Wiley & Sons) 🌐 English ⚖ 847 KB

## Abstract The noninteracting local‐structure model of the folding and unfolding transition in globular proteins, the formulation of which was given in the preceding paper, is applied to the analysis of the two‐dimensional lattice model of proteins. The lattice model of proteins is a theoretical t

Application of Schmidt's method to the c

Application of Schmidt's method to the calculation of spalding's function and of the skin-friction coefficient in turbulent flow

✍ J. Kestin; L.N. Persen 📂 Article 📅 1962 🏛 Elsevier Science 🌐 English ⚖ 730 KB

Characterization and determination of ti

Characterization and determination of titratable groups of proteins by linearization of titration curves. II. Application to lysozyme

✍ O.E.S. Godinho; L.M. Aleixo; J.P. Hora Alves 📂 Article 📅 1982 🏛 Elsevier Science 🌐 English ⚖ 425 KB

The potentiometric acid-base titration curve of fully protonated lysozyme at ionic strengths of 0. IO and f .O M has been performed. The stoichiometry and the pK\* values of each titratable group have been determined through the linearization of titration curves. Two types of carboxylic groups with