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Frequency of nonsense mutations (amber and ochre) induced by UV light in the ura3 gene in Saccharomyces cerevisiae

โœ Scribed by P. Gerola; S. Sora


Book ID
114071001
Publisher
Elsevier Science
Year
1975
Tongue
English
Weight
194 KB
Volume
30
Category
Article
ISSN
0027-5107

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A forward mutation system has been developed to obtain rapidly clonable mutants at the URA3 locus in yeast by means of selection for 5-fluoroorotic acid resistance. We have used this system to determine base changes in 35 spontaneous and 34 ultraviolet radiation-induced ura3 base substitution mutant