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Frequency of cells expressing CD44, a Head and Neck cancer stem cell marker: Correlation with tumor aggressiveness

✍ Scribed by Benzion Joshua; Michael J. Kaplan; Ilana Doweck; Reetesh Pai; Irving L. Weissman; Mark E. Prince; Laurie E. Ailles

Publisher
John Wiley and Sons
Year
2011
Tongue
English
Weight
270 KB
Volume
34
Category
Article
ISSN
1043-3074

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✦ Synopsis

Abstract

Background

We previously identified by flow cytometry a Lineage‐CD44+ (Lin‐CD44+) subpopulation of cells with cancer stem cell properties in head and neck squamous cell carcinoma (HNSCC). We now correlate clinical and histologic factors with Lin‐CD44+ cell frequency.

Methods

The study included 31 patients with HNSCC, of whom 87% had stage IV disease. The frequency of Lin‐CD44+ cells and the success of xenografting patient tumors in mice were correlated with clinical and pathologic data.

Results

The mean frequency of Lin‐CD44+ cells was 25% (0.4%–81%). It was 36% in patients who had recurrence versus 15% for those without recurrence (p = .04). Successful xenograft implantation occurred in 53%. Seventy‐five percent of patients with successful xenografts had recurrence versus 21% of patients with unsuccessful xenografts (p = .003).

Conclusions

Successful xenograft implantation and a high frequency of Lin‐CD44+ cells correlate with known poor prognostic factors such as advanced T classification and recurrence. These findings may support the stem cell concept in HNSCC. Β© 2011 Wiley Periodicals, Inc. Head Neck, 2012

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