Frequency-modulation SFM imaging of DNA molecules in UHV conditions
✍ Scribed by Maeda, Yasushi; Matsumoto, Takuya; Kawai, Tomoji
- Book ID
- 102647800
- Publisher
- John Wiley and Sons
- Year
- 1999
- Tongue
- English
- Weight
- 547 KB
- Volume
- 27
- Category
- Article
- ISSN
- 0142-2421
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✦ Synopsis
Dynamic mode scanning force microscopy with frequency-shift detection has been employed for imaging singleand double-stranded DNA under ultrahigh vacuum conditions. For the wet processes used to prepare the samples, the strong adhesion force at the surface is observed even in ultrahigh vacuum conditions. The DNA images can only be obtained with large frequency shifts, but provide sufficient resolution to see the coil or higher-order structures of the DNA molecules in detail. Because of the high sensitivity of the tip-sample interaction, frequency-shift detection mode provides a higher quality grey-scale resolution than tapping mode. In addition, contrast artefacts, which might include information on the detailed structure, are often observed, depending upon the feedback conditions.