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Frequency domain measurements of the fluorescence lifetime of ribonuclease T1

✍ Scribed by Eftink, M.R.; Ghiron, C.A.

Book ID
118518889
Publisher
Biophysical Society
Year
1987
Tongue
English
Weight
800 KB
Volume
52
Category
Article
ISSN
0006-3495

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## Abstract Frequency‐domain fluorescence lifetime imaging microscopy (FLIM) has become a commonly used technique to measure lifetimes in biological systems. However, lifetime measurements are strongly dependent on numerous experimental parameters. Here, we describe a complete calibration and chara