𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Frequency analysis of infrared absorption and vibrational circular dichroism of proteins in D2O solution

✍ Scribed by Petr Pancoska; Lijiang Wang; Timothy A. Keiderling

Book ID
105356108
Publisher
Cold Spring Harbor Laboratory Press
Year
2008
Tongue
English
Weight
848 KB
Volume
2
Category
Article
ISSN
0961-8368

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

The IR absorption frequencies as derived from second derivatives of the Fourier transform IR spectra of the amide I' bands of globular proteins in D~2~O are compared to those obtained from band fitting of the vibrational circular dichroism (VCD) spectra. The two sets of frequencies are in very good agreement, yielding consistent ranges where amide I' VCD and IR features occur. Use of VCD to complement the IR allows one to add sign information to the frequency information so that features occurring in the overlapping frequency ranges that might arise from different secondary structures can be better discriminated. From this comparison, it is clear that correlation just of the frequency of a given IR transition to secondary structure can lead to a nonunique solution. Different sign patterns were identified for correlated groups of globular proteins in restricted frequency ranges that have been previously assigned to defined secondary structural elements. Hence, different secondary structural elements must contribute band components to a given frequency range.

πŸ“œ SIMILAR VOLUMES

The structure of antimicrobial pexiganan
The structure of antimicrobial pexiganan peptide in solution probed by Fourier transform infrared absorption, vibrational circular dichroism, and electronic circular dichroism spectroscopy
✍ Ganesh Shanmugam; Prasad L. Polavarapu; D. Gopinath; R. Jayakumar πŸ“‚ Article πŸ“… 2005 πŸ› Wiley (John Wiley & Sons) 🌐 English βš– 103 KB πŸ‘ 2 views

## Abstract Pexiganan (Gly–Ile–Gly–Lys–Phe–Leu–Lys–Lys–Ala–Lys–Lys–Phe–Gly–Lys–Ala–Phe–Val–Lys–Ile–Leu–Lys–Lys), a 22 amino acid peptide, is an analogue of the magainin family of antimicrobial peptides present in the skin of the African clawed frog. Conformational analysis of pexiganan was carried

Conformational analysis of melittin in s
Conformational analysis of melittin in solution phase: Vibrational circular dichroism study
✍ Feng Wang; Prasad L. Polavarapu πŸ“‚ Article πŸ“… 2003 πŸ› Wiley (John Wiley & Sons) 🌐 English βš– 200 KB πŸ‘ 2 views

## Abstract The vibrational absorption and vibrational circular dichroism (VCD) spectra of melittin in D~2~O solutions at different pH values, different salt concentrations, or different 2,2,2‐trifluoroethanol (TFE) concentrations are recorded in the amide Iβ€² (1850–1600 cm^βˆ’1^) region. Two models a

[Advances in Protein Chemistry] Unfolded
[Advances in Protein Chemistry] Unfolded Proteins Volume 62 || Unfolded peptides and proteins studied with infrared absorption and vibrational circular dichroism spectra
✍ Keiderling, Timothy A. πŸ“‚ Article πŸ“… 2002 πŸ› Elsevier 🌐 English βš– 816 KB

A variety of complementary techniques and approaches have been used to characterize peptide and protein unfolding induced by temperature, pressure, and solvent. **Volume 62, Unfolded Proteins**, assembles these complementary views to develop a more complete picture of denatured peptides and proteins