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Freezing of zona-free mouse embryos: Characteristics of the plasma membrane and subsequent development of the embryos

✍ Scribed by Martí, M. ;Nogués, C. ;Egozcue, J. ;Ponsà, M.

Publisher
John Wiley and Sons
Year
1995
Tongue
English
Weight
824 KB
Volume
272
Category
Article
ISSN
0022-104X

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✦ Synopsis

Abstract

Frozen‐thawed mouse embryos with (+ZP) and without (−ZP) zona pellucida have been studied at the Scanning Electron Microscope (SEM) to determine how the process affects the plasma membrane and the subsequent embryo development. The main difference observed in −ZP embryos immediately after thawing is the abnormal morphology and distribution of microvilli. This could explain the spontaneous separation of blastomeres in ‐ZP embryos, and the decrease in their survival rate. If thawed −ZP embryos are allowed to recover in culture, their plasma membrane characteristics and survival rate are identical to those of control embryos. © 1995 Wiley‐Liss, Inc.

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