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Freezing of rabbit embryos in the presence of methanol

✍ Scribed by K. Papis; J. Dziak

Book ID: 115851018
Publisher: Elsevier Science
Year: 1988
Tongue: English
Weight: 88 KB
Volume: 25
Category: Article
ISSN: 0011-2240
DOI: 10.1016/0011-2240(88)90527-5

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## Abstract Eight‐cell mouse embryos were frozen by using erythritol as the cryoprotective agent. The samples were cooled slowly (1°C/min) to temperatures between − 15 and − 75°C before direct transfer into liquid nitrogen (− 196°C). The most effective concentration of erythritol for freezing of em