✦ LIBER ✦

Freeze-drying method for stable preservation in cellular specimens for the detection of apoptotic cells

✍ Scribed by Takazo Okuyama; Shinji Matsuo; Daiji Nagamachi; Kiyotugu Yoshikawa; Tadao Kobayashi; Rei Takahashi; Sakan Maeda

Publisher: John Wiley and Sons
Year: 2000
Tongue: English
Weight: 66 KB
Volume: 22
Category: Article
ISSN: 8755-1039
DOI: 10.1002/(sici)1097-0339(200004)22:4<254::aid-dc12>3.0.co;2-p

No coin nor oath required. For personal study only.

✦ Synopsis

We describe a method of cytologic specimens for the detection of apoptotic cells by immunostaining after long-term storage (Ͼ6 mo) using TdT-mediated dUTP-biotin nick end labelling (TUNEL) technique. The application of the TUNEL method on freeze-drying cellular sample will eliminate the need for fresh material and will provide several advantages.