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Free radical scavenging activity of coenzyme Q measured by a chemiluminescent assay

✍ Scribed by Maurizio Battino; Elida Ferri; Stefano Girotti; Giorgio Lenaz

Publisher
Elsevier Science
Year
1991
Tongue
English
Weight
393 KB
Volume
255
Category
Article
ISSN
0003-2670

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✦ Synopsis

Involvement of coenzyme Q (CoQ) m anti-omdant actiwhes, m ad&bon to Its mayor redox role, has frequently been suggested m recent years In order to eluudate it CoQ could really be engaged m scavengmg free rticals produced endogenously m a blologtcal system, an expenmental system was developed m which beef heart rmtcchondna m the presence of a saturatmg NADH concentra&m and of rotenone produce free rad&s The presence of oxygen-reactwe forms was easdy detected by a hunmol-depen~t chemdummescence process The chemdummcacence assay showed that short-&am CoQ homologues can act as pro-oudants, enhancmg free r&Cal effects, whde exogenous coenzyme Q10 could scavenge free r&cals, especially at very low concentration In tis system, exogenous CM&,-, was more effecuve than a-tocopherol at the same concentratmn m scavengmg free ra&cals The molecular mechamsm that leads to dus act~ty 1s stdl unclear, but these results are of blcchemtcal and blomedrcal importance because they m&cate that CoQ may act as an an&ox&mt m situations mmuckmg physmpathologtcal con&tions This dmzct cheuul~escent method w protmsmg for stties of bmchermcal processes which mvolve acuve oxygen specnzs Keyworrdr Chemd~escence, Coewyme Q

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