Free carboxylate groups required for transport of neutral amino acids by the Ehrlich ascites-tumor cell
β Scribed by Javier Garcia-Sancho; Ana Sanchez; Halvor N. Christensen
- Book ID
- 115723468
- Publisher
- Elsevier Science
- Year
- 1977
- Tongue
- English
- Weight
- 144 KB
- Volume
- 465
- Category
- Article
- ISSN
- 0005-2736
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## Abstract Solubilized protein fractions have been obtained from plasma membranes of Ehrlich ascites cells either by extraction with 0.5% Triton Xβ100 or by extraction with 2% cholate. Partial purification of the solubilized protein fraction has been obtained by utilizing a combination of ammonium
## Abstract Recent investigations have indicated that cellular rheogenic properties may interfere with the correct estimation of Na^+^ and amino transport stoichiometry. We have reevaluated the stoichiometry of Na^+^ and Ξ±βaminoisobutyric acid (Ξ±βAIB) cotransport in Ehrlich ascites tumor cells depl
Using the double thymidine block technique, Ehrlich ascites tumor cells (ELD) carried i n continuous spinner culture have been synchronized. Simultaneous monitoring of :tH-thymidine incorporation, cell number and mitotic index yielded a cell cycle time of approximately 13.5 hours. This is composed o