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Fragmentation characteristics of peptide–metal ion adducts under matrix-assisted laser desorption/ionization post-source decay time-of-flight mass spectrometric conditions

✍ Scribed by Dhammika Jayawardene; Chhabil Dass

Publisher
John Wiley and Sons
Year
2002
Tongue
English
Weight
116 KB
Volume
37
Category
Article
ISSN
1076-5174

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✦ Synopsis

Abstract

Fragmentation reactions of sodium‐cationized enkephalin peptides generated by matrix‐assisted laser desorption/ionization were studied using post‐source decay (PSD) with a reflectron time‐of‐flight mass spectrometer. Several matrices and analyte–matrix sample preparation methods were evaluated for high‐intensity ion currents that could last for the entire PSD analysis. A triple dried‐droplet sample preparation procedure with 2,5‐dihydroxybenzoic acid as the matrix was found to yield abundant longer‐lasting ion signals of the peptide–Na^+^ ion adducts. The principal decay product of these adduct ions is the [b~n−1~ + Na + OH]^+^ ion, which provides an unambiguous identification of the C‐terminal residue of a peptide. In some peptides, the loss of a second residue from the C‐terminus is also observed. No other sequence‐specific ions were observed. Copyright © 2002 John Wiley & Sons, Ltd.

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